英语翻译ResultsDetectionofupregulatedproteinsInordertoidentifymembraneproteinsofK562cellswithincreasedexpressionunderirondeprivation,weisolatedhydrophobicmembraneproteinsfromthecellsafter24-hincubationundercontrolcondit

　　英语翻译

　　Results

　　Detectionofupregulatedproteins

　　InordertoidentifymembraneproteinsofK562cellswith

　　increasedexpressionunderirondeprivation,weisolated

　　hydrophobicmembraneproteinsfromthecellsafter24-h

　　incubationundercontrolconditions(definedtransferrin

　　mediumsupplementedwith5lg/mloftransferrinasthe

　　sourceofiron)andunderirondeprivation(definedironfree

　　mediumwithoutanyaddedsourceofiron,seeMaterials

　　andmethods)usingatwo-phasepartitioningsystem

　　(2-DSamplePrepforMembraneProteinsfromPierce,see

　　Materialsandmethods).Wepreparedthreepairsof2Dgels

　　fromthreeseparatepairsofsamplesofcontrolcellsand

　　cellsculturedunderirondeprivation.Gelswerestained

　　usingcolloidalCoomassieblue.Afterscanningthestained

　　gelsinadensitometer,weanalyzedtripletsofgelimages

　　usingPDQuest7.12-DAnalysisSoftwareinsemimanual

　　detectingandmatchingmode.

　　Approximately300stablespotsweredetectedoneachgel

　　map.TheoverallproteinexpressionprofileswithpI3–11

　　andmolecularmassof20–120kDawereverysimilarwithin

　　thethreegelsofbothgroupsofsamples(controlandiron

　　deprivation).Aftertheelectronicconstructionofavirtual

　　Mastergel,wesearchedforspotsrepresentingproteinswith

　　significantlyincreasedexpressionunderirondeprivation

　　whencomparedwiththeexpressionundercontrolconditions.

　　Theincreasedexpressionunderirondeprivationwas

　　showntobestatisticallysignificantinthecaseoftwospots,

　　i.e.,spot1(P.05)andspot2(P.01).Positionsof

　　thesetwospotsonrepresentative2DgelsareshowninFig.1

　　andtheeffectofirondeprivationontheexpressionofproteins

　　ofthesespotsisshowninFig.2.